infinity-corrected tube lens ttl200  (Thorlabs)

Journal: Nature protocols

Article Title: Near-infrared catecholamine nanosensors for high spatiotemporal dopamine imaging

doi: 10.1038/s41596-021-00530-4

Figure Lengend Snippet: a, Schematic representation of the dissection process for the generation of acute coronal brain slices. Red dashed lines represent areas to be cut by using scissors or a vibratome. b Incubation set up to label acute brain slices with nIRCat. c, Microscope perfusion bath set up to image nIRCat-labeled acute brain slices. Coronal brain slices should be placed in the perfusion bath (circle 1) and weighed down by a harp. Acute slices should be placed with the nIRCat-labeled side facing toward the microscope objective.

Article Snippet: Infinity-corrected tube lens for custom microscope builds (e.g., Thorlabs, TTL200-S8).

Techniques: Dissection, Incubation, Microscopy, Labeling

Journal: Nature protocols

Article Title: Near-infrared catecholamine nanosensors for high spatiotemporal dopamine imaging

doi: 10.1038/s41596-021-00530-4

Figure Lengend Snippet: a, Schematic depicting the arrangement of optical components in an nIRCat imaging system. Laser light is beam-expanded and combined into a multimode optical fiber and then focused to the back focal plane of an nIR-compatible objective. Fluorescence emitted by the sample is re-collected and passed through 900-nm long-pass filters and additional cleanup filters. The transmitted fluorescence is focused by a tube lens onto the InGaAs camera sensor. b, Schematic depicting the microscope control and automation of time series image acquisitions and stimulations of acute brain slices. c, Representative nIR image of dorsal striatum obtained from an nIRCat-labeled mouse acute brain slice. Square ROIs depict regions where baseline fluorescence was above a threshold value. Squares outlined in red exhibited a negligible fluorescence transient after stimulation, whereas those outlined in green were identified as ‘active’ regions with a statistically significant transient associated with dopamine release. Scale bar = 20 μm. d, Representative brightfield (BF) image and nIR fluorescence images showing the increase in relative fluorescence (ΔF/F) in response to dopamine release evoked by an electrical stimulation (red triangle). Scale bar = 10 μm. e, ΔF/F trace averaged over all ‘active’ regions (orange) with standard deviation bounds (gray). f and g, Representative results demonstrating that nIRCat nanosensor fluorescence reflects the changes to dopamine release induced by 1 μM D2 agonist quinpirole f and D2 antagonist sulpiride (g). h, A histogram depicting the regional heterogeneity of sulpiride’s impact on dopamine release for individual ROIs (~2-μm diameter). BE, beam expander, DCMLP, dichroic long-pass mirror; LP, long-pass filter; OF, optical fiber; TL, tube lens. Plots in f–h were adapted from ref. 19.

Article Snippet: Infinity-corrected tube lens for custom microscope builds (e.g., Thorlabs, TTL200-S8).

Techniques: Imaging, Fluorescence, Microscopy, Control, Labeling, Slice Preparation, Standard Deviation

Journal: Nature protocols

Article Title: Near-infrared catecholamine nanosensors for high spatiotemporal dopamine imaging

doi: 10.1038/s41596-021-00530-4

Figure Lengend Snippet: Troubleshooting table

Article Snippet: Infinity-corrected tube lens for custom microscope builds (e.g., Thorlabs, TTL200-S8).

Techniques: Concentration Assay, Sonication, Synthesized, Suspension, Generated, Dissection, Imaging, Labeling, Transferring, In Vitro, Microscopy, Slice Preparation